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Flow cytometric analysis of Anti-BCMA(C11D5.3) CAR-293 cells staining with Alexa Fluor 555-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (Cat. No. C13-AEFMY2) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with Isotype control antibody. Alexa Fluor 555 signal was used to evaluate the binding activity (QC tested).
Non-specificity of Alexa Fluor 555-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (Cat. No. C13-AEFMY2) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC anti-CD3 antibody and Alexa Fluor 555-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and Alexa Fluor 555 positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
The purity of Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (Cat. No. C13-MY2010b) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Immobilized Mouse anti-BCMA scFv (C11D5.3), His Tag at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (Cat. No. C13-MY2010b) with a linear range of 0.2-8 ng/mL (QC tested).
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